Extended Data Fig. 6: Representative desensitization and rectification traces.
a, Representative desensitization traces of GluK2 and mutants responded to 60 ms application of 10 mM glutamate were normalized and aligned to the peak. Superimposed responses of the receptor alone and the receptor-Neto2 complex were shown in black and green traces, respectively. b, Normalized current-voltage relationship of GluK2 mutants in the absence and presence of Neto2. n value represents independent cells for analysis. c, Representative desensitization traces and related statistical analysis (GluA2, n = 16; GluA2K2ICD, n = 12). Each symbol represents a single cell recording, and n value represents biologically independent cells for statistical analysis. Significances were determined using two-sided unpaired t-test. Not significant (ns), P = 0.0755. No adjustments were made for multiple comparisons. Error bars stand for S.E.M. The H1-helix is composed of residues 857FCSAMVEELRMSLK870 and removed in GluK2ΔH1 construct. The amino acid sequence of the ICD of GluK2 and GluA2 between M1-M2 helices are 587YEWYNPHPCNPDSDVVEN604 and 570YEWHTEEFEDGRETQSSESTNE591, respectively, which are involved in the ICD swapping constructs of GluA2K2ICD and GluK2A2ICD. d, I-V relationship for GluA2, GluK2 and related mutants. Desensitization curves (10 mM glutamate for 200 ms) were recorded at holding potential ranging from −100 to +100 mV in 20 mV increasement. Traces were normalized to the peak value at −100 mV.
