Extended Data Fig. 7: Interactions stabilizing the pore helix M2.

a, EM density map of the LBD and TMD layers of the GluK2-1×Neto2 complex. Subunits A/C and B/D of GluK2 are colored in blue and red, respectively. The Neto2 protein is colored in orange. N-glycans are colored in yellow. b, The ion conduction pore and its profile of the GluK2-Neto2 complex. Pore loops are colored in green. A cation ion is shown as a grey sphere, overlaid with corresponding EM density colored in marine. Q621, T652, A656, and T660 are shown in sticks. Constriction sites are indicated in the pore profile. c, The TM helices of the GluK2 (red and blue) and the Neto2 (yellow) are shown as cartoon. The EM density of M2 helix are shown as transparent grey surface. Critical residues involved in interactions are shown as sticks. d, “Top-down” view of M2 helices and the pore loops. Q621 residues are shown in sticks.