Extended Data Fig. 5: Anterograde bulk AAV tracing of projections from Gnb4+ neurons in claustrum or lateral cortex.
From: Morphological diversity of single neurons in molecularly defined cell types
a–e, AAV2/1-pCAG-FLEX-GFP tracer was injected into the claustrum (a, b), SSs (c, d) or SSp (e) in Gnb4-IRES2-Cre or Gnb4-IRES2-CreERT2 mice. Brains were imaged by the TissueCyte STPT system. First panel in each row: top-down view of segmented GFP-labeled axon projections in the cortex. Second panel: injection site. Third panel: the fine axon fibers in a target cortical area. Fourth panel: the segmented image of the third panel to visualize and quantify the axon fibers. Fifth panel in a and b: axon fibers observed in basolateral amygdalar nucleus (BLA). Full STPT image datasets are available at the Allen Mouse Brain Connectivity Atlas web portal (http://connectivity.brain-map.org/) with the following experiment IDs: a, 514505957; b, 485902743; c, 553446684; d, 581327676; e, 656688345. These 5 selected datasets were replicates of each other and all had small, spatially specific, injection sites that were located very close to each other. These small bulk injections demonstrate very distinct projection patterns between claustral and cortical Gnb4+ neurons.
