Extended Data Fig. 6: SOX5 directly suppresses RXRG-RARB responsive human, chimpanzee, gorilla and mouse Cbln2 E2 enhancer.
From: Hominini-specific regulation of CBLN2 increases prefrontal spinogenesis
a, Luciferase assay in Neuro-2a cell line from luciferase reporters conjugated to chimpanzee and gorilla wild CBLN2 E2. Two-tailed Student’s t-test; *P = 0.01; ***P = 9e-5 (Chimpanzee), 1e-5 (Gorilla); NS, not significant. Error bars; S.E.M.; N = 3 per condition. b, Overexpression of human SOX5 exerts a similar effect to mouse Sox5 on human and mouse Cbln2 E2 reporters. Two-tailed Student’s t-test; *P = 5e-3; ***P = 4e-5; ****P = 2e-6; 2e-6, 1e-6 (Human SOX5 + mouse Cbln2 E2); NS, not significant; Error bars, S.E.M.; N = 3 per condition. c, Cbln2 expression is upregulated in Sox5 conditional knockout brain at PD 0. Additional two replicates (Repl #2 and 3) not shown in Fig. 2d are shown. Scale bar, 200 μm (left); 100 μm (right). d, Constructs used for luciferase assay and generation of transgenic animals shown in e, f. e, Transgenic mouse brain at PCD 17 carrying Cbln2 E2 (N = 3) or Cbln2 E2 Fr1-lacZ reporters (N = 3). Cbln2 expression in the PFC is indicated by arrowheads. Endogenous Cbln2 expression is also shown for comparison (N = 4). Scale bar, 200 μm. f, Luciferase assay for Cbln2 E2 and Cbln2 E2-Fr1. Two-tailed Student’s t-test; **P = 1e-4 (Cbln2 E2), 2e-4 (Cbln2 E2-Fr1); ***P = 8e-5; NS, not significant; Error bars: S.E.M.; N = 3 per condition.
