Extended Data Fig. 8: Additional replicates.
From: Hominini-specific regulation of CBLN2 increases prefrontal spinogenesis
Additional replicates from Fig. 3. a, b, The neocortex of the humanized hCbln2 E2 knock-in prenatal and neonatal mice exhibits upregulated Cbln2 in both upper and deeper layers compared to mice carrying WT Cbln2 E2. Additional two replicates (Repl #2 and 3) not shown in Fig. 3a are shown for PND 16 and PND18. Scale bars, 200 μm (left); 100 μm (right). All analysis and three replicates for PD1. Neocortex was divided into six equal bins spanning from pia to ventricular zone, and Cbln2 signal intensity was quantified for each bin and compared between WT and hCbln2 E2 (Hs;Hs). Two-tailed Student’s t-test; *P = 0.02 (PCD18), 0.02, 0.04 (PD1), **P = 1e-3 (PCD18), 1e-3, 3e-3, 3e-3 (PD1), ***P = 1e-4, 2e-4 (PCD18); N = 3 per genotype. Scale bars, 100 μm. c, Expression of the upper layer marker, Cux2, and SOX5 in adjacent tissue sections were detected by in situ hybridization and immunostaining, respectively. Scale bars, 100 μm. N = 3.
