Extended Data Fig. 10: Quantification of excitatory and inhibitory postsynaptic puncta.
From: Hominini-specific regulation of CBLN2 increases prefrontal spinogenesis
a–d, PD 0 WT and hCbln2 E2 mPFC L2-3 and L5 (BCL11B-immunopositive) immunostained for PSD-95/DLG4 or GPHN. Density of PSD-95/DLG4+ excitatory and GPHN+ inhibitory postsynaptic puncta in PD 0 and PD 60 mPFC, primary somatosensory area (SSp), and primary visual area (VISp) of WT (blue), hCbln2 E2 (orange), and Sox5 cKO mice (green). Two-tailed Student’s test; *P = 0.018; ***P = 4e-4, 1e-4 (b, WT vs. humanized hCbln2 E2 L2-3, and L5, respectively), 1e-4, 7e-5, 4e-4, 2e-4 (d, WT vs. humanized hCbln2 E2 L2-3 and L5, respectively; WT vs. Sox5 cKO L2-3 and L5, respectively); ****P < 1e-5. Error bars: S.E.M.; N = 5 per PD 0 genotype; N = 3 per PD 60 genotype. Scale bar, 25 μm.
