Extended Data Fig. 10: RA signal in the neonatal mouse forebrain.
From: Regulation of prefrontal patterning and connectivity by retinoic acid
a, β-galactosidase histochemical staining of more posterior regions of Rarb+/+; Rxrg+/+ (WT); RARE-lacZ and Rarb-/-; Rxrg-/- (dKO); RARE-lacZ mouse brains at PD 0. Signal intensity in the boxed area (ACA, SSp, RSPA, STR, HIP and VP) was quantified. Note the reduced activity in anteromedial structures including ACA and RSPA (RSP). There is also reduced expression in HIP and lateral STR, but not the thalamus. Two-tailed Student’s t-test; *P = 9e-3, **P = 7e-4; 3e-3 (from left), ***P = 2e-4, ****P = 2e-7; 1e-5; 4e-5; 5e-6; 6e-6 (from left). N = 3 per genotype: Errors bars: S.E.M.; Scale bars, 200 μm. b, Cbln2 and Meis2 expression in PD 0 WT and dKO mutant brain by in situ hybridization at PD 0. Note that Cbln2 and Meis2 expression in mPFC was decreased in dKO. Scale bar, 200 μm. N = 3 per genotype. ACA, Anterior cingulate area; CP, caudoputamen; HIP, Hippocampus; RSPA/RSP, Retrosplenial area; STR, striatum; VP, Ventroposterior thalamus.
