Extended Data Fig. 12: Additional analysis of RNA-seq experiments.

a, Number of upregulated genes between PD 0 WT and dKO mice per region and phenotype, as well as combinations of regions and phenotypes. b, Gene loadings of the first principal component from PCA in Fig. 3. Colours represent the frontal cortex region where the gene was found to be upregulated. c, Cellular component GO terms associated with the total list of 4,768 DEx genes found, showing their z-score and nominal P values. Z-score represents the proportion of upregulated versus downregulated genes in the list of DEx genes associated to each GO term (i.e. z-score = (#up - #down) / sqrt (#all DEx associated to the GO term)). Dark blue: z-score <−5; light blue: z-score (−5,0]; orange: z-score >0. Size of the bubbles are proportional to the total number of DEx genes associated to the given GO term. d, Cellular component GO terms associated with DEx genes found in all three frontal cortex regions, and DEx genes unique to each region (mPFC, OFC, MOs) and their unadjusted p value. e, Enrichment of RA related genes (green), genes upregulated in individual lobes of the mid-fetal human cortex based on Fig. 1 (purple), and psychiatric disease related genes in up- and downregulated genes (pink). DEx genes are separated by genes that are DEx only in the given region (mPFC, OFC, MOs), genes that are DEx in the two given regions (mPFC + OFC, mPFC + MOs, OFC + MOs), genes that are DEx in all three regions (mPFC + OFC + MOs). Circles plotted for significant enrichments (P value <0.05), in darker colour, significance is considering the adjusted p value. Diameter of circle is associated with odds ratio per legend.