Extended Data Fig. 13: Altered synaptic density and axonal projections in Rxrg and Rarb dKO mice.
From: Regulation of prefrontal patterning and connectivity by retinoic acid
a, Example of downregulated genes between PD 0 Rarb+/+; Rxrg+/+ (WT) and Rarb-/-; Rxrg-/- (dKO) that displayed an anterior to posterior gradient in PCD 18 mouse embryo (images are from the Allen Developing Mouse Brain Atlas; developingmouse.brain-map.org/58). Scale bar: 1 mm. b, Immunostaining for PSD95/DLG4 in the cortical subregions (mPFC, MOs, OFC, MOp, and SSp) of WT and dKO brain at PD 0. Each region as shown in Fig. 2c. Scale bar: 25 μm. N = 3 per genotype. c, Quantification and representative images of co-localized synaptophysin (SYP) and PSD95 puncta in PD 0 WT and dKO mPFC. Two-tailed Student’s t-test; *P = 0.01; N = 3 per genotype; Errors bars: S.E.M.; Scale bar: 10 μm. d, e, DiI placement in mPFC (c) and medial thalamus (d) with tracing data in WT and dKO brain at P21. Additional two replicates of experiment shown in Fig. 4d, e are shown. Asterisks: DiI crystal placement. Scale bar: 1 mm.
