Extended Data Fig. 16: Analysis of axonal projections and cell death in Rarb and Rxrg dKO mice.

a, Four scalar indexes which describe microstructural integrity do not differ in the four major white-matter tracts (corpus callosum, anterior commissure, left and right internal capsules) between WT and dKO mice. FA Comparisons: Corpus callosum (two-tailed unpaired t-test, P = 0.4); Anterior cingulate (two tailed mann-whitney test, P = 0.2); Internal Capsule left (two-tailed unpaired t-test, P = 0.07); Internal Capsule right (two-tailed unpaired t test, P = 0.8). ADC Comparisons: Corpus callosum (two-tailed unpaired t-test, p value 0.1); Anterior cingulate (two-tailed unpaired t-test, p value 0.1); Internal Capsule left (two-tailed unpaired t-test, p value 0.9); Internal Capsule right (two-tailed unpaired t-test, P = 0.3). RD Comparisons: Corpus callosum (two-tailed unpaired t-test, P = 0.1); Anterior cingulate (two-tailed unpaired t-test, P = 0.1); Internal Capsule left (two tailed mann-whitney test, P = 0.1); Internal Capsule right (two-tailed unpaired t-test, P = 0.3). AD Comparisons: Corpus callosum (two-tailed unpaired t-test, P = 0.08); Anterior cingulate (two-tailed unpaired t-test, P = 0.1); Internal Capsule left (two-tailed unpaired t-test, P = 0.4); Internal Capsule right (two-tailed unpaired t-test, P = 0.2). N=5; Errors bars: S.E.M. b, Number of streamlines of MOp-thalamus and corticocortical tracts, did not differ between WT and dKO. Two-tailed unpaired t-test for MOp-MOp, MOp-Th, and AUDp-th (P = 0.1, 0.07, and 0.6, respectively). Two-tailed Mann-Whitney test for ACA-Th (P = 0.3). N = 5 per genotype; Errors bars: S.E.M. c, Barrel formation was examined by Nissl staining at PD 5 in WT and dKO. N = 3 per genotype. d, Representative images and quantification of corticospinal tract (CST) width using L1CAM expression at PD 5 in WT and dKO. The width of the corticospinal tract (shown in brackets) is slightly increased in dKO. Two-tailed Student’s t-test: *P = 0.02; N = 5 per genotype; Errors bars: S.E.M. e, Representative images and quantification of of apoptotic cells in the mPFC detected by cleaved caspase3 (actCASP3) between WT and dKO. Two-tailed Student’s t-test: WT vs. dKO; NS; N = 5 per genotype; Errors bars: S.E.M.; Scale bars: 500 μm; 200 μm (b,f); 1 mm (c); 100 μm (d). f, Representative images and quantification of tyrosine hydroxylase (TH) immunolabelled axons in WT and dKO mouse frontal cortex at PD 5 (N = 4). Rectangles represent regions analysed. Clockwise starting from left-most: mPFC, MOs, OFC. Errors bars: S.E.M.