Extended Data Fig. 18: RA signal in posterior cortical regions of Cyp26b1 KO mice.
From: Regulation of prefrontal patterning and connectivity by retinoic acid
a, Strategy for the generation of Cyp26b1-/- (Cyp26b1 KO) mice using CRISPR-Cas9 gene editing technique41. b, Cyp26b1 expression in PD 0 mice cortex by in situ hybridization. The colorimetric staining was purposefully extended compared to the experiment in Fig. 5a to better visualize low expressing locations. c, β-Galactosidase staining of more posterior regions of control Cyp26b1+/+; RARE-lacZ (Ctrl) and Cyp26b1-/-; RARE-lacZ (KO) mouse brains at PCD 18. Scale bar: 500 μm. Intensity of signal in the boxed areas (ACA, MO, SSp) was quantified. Increase in RA signalling in Cyp26b1 KO brains is less significant in posterior regions. Two-tailed Student’s t-test: Ctrl vs. Cyp26b1 KO: *P = 0.01; 0.04; 9e-3; 7e-3; 6e-3; 0.02; 0.02; **P = 4e-3; 1e-3; 4e-3; 4e-3, N = 3 per genotype; Errors bars: S.E.M.; Scale bars: 200 μm.
