Extended Data Fig. 19: Rorb expression and medial thalamocortical projections in neonatal Cyp26b1 KO mice.

a, Additional two replicates of Rorb expression in WT and Cyp26b1 KO brains from Fig. 5f are shown. Scale bar: 100 μm. b, DiI was placed in the medial thalamus of WT and Cyp26b1 KO brains, and signal was detected in the PFC. Additional two replicates of experiment in Fig. 5d are shown. N = 3 per genotype and condition. Arrowheads: Thalamocortical innervation of the medial and dorsolateral frontal cortex. Asterisks: DiI crystals placed. Scale bar: 400 μm. c, DiI was placed in the frontal motor cortex of WT and Cyp26b1 KO brains, and signal was detected in the medial thalamus. Additional two replicates of experiment in Fig. 5e are shown. N = 3 per genotype and condition. Of note, owing to restriction related to Covid-19, PD 0 brains were left 2 months in cold room after 3 weeks in 37 degree. Scale bar: 400 μm.