Extended Data Fig. 5: dCA2-projecting MS cells are primarily cholinergic and glutamatergic.
From: 5-HT modulation of a medial septal circuit tunes social memory stability
a, Schematic of monosynaptic rabies tracing set-up in Amigo2-Cre mice (left) and representative images of injection site in the dCA2 as well as Gfp-positive cells in the MS (right). Scale bar, 200 μm, n = 3. b, Representative images of in-situ hybridization. Scale bar, 200 μm (upper left panel), 40 μm. c, Quantification of in-situ hybridization, n = 3 subjects. d, Quantification of same in-situ hybridization data for percent of Gfp-positive cells colocalizing with Chat only, Slc17a6 only, Gad2 only or double-positive for Slc17a6 and Chat, Gad2 and Chat (right), n = 3 subjects. e, Quantification of colocalization with GFP-positive cells via immunohistochemistry (IHC) using either CHAT or CaMKII antibodies (left) and representative images (right, scale bar, 40 μm, n = 3). f, Schematic of experimental set-up and representative image of dCA2 infusion site filled with ink. g, Duration in chamber with no or nm (left) and discrimination scores (right) (F2,36 = 1.702, P = 0.1967; n = 13). h, Duration in chamber with fo or no (left) and discrimination scores (right) (F2,36 = 3.259, P = 0.05; n = 13). Statistical tests: g, h, duration: two-tailed paired Student’s t-test; discrimination scores: one-way ANOVA with Tukey’s post-hoc test. N.S. = not significant, *P < 0.05, **P < 0.01, ***P < 0.001. Error bars denote s.e.m.
