Extended Data Fig. 6: MS synapses onto dCA2 pyramidal neurons are primarily glutamatergic and can express long-term depression ex vivo.
From: 5-HT modulation of a medial septal circuit tunes social memory stability
a, Top, pie charts of percentage of cells, in which the ChETA (n = 20/9) and ChR2 (n = 22/6) induced PSCs were >60% reduced by NBQX (10 μM), picrotoxin (50 μM) or Mecamylamine (5 μM). Below, summary time course of ChETA-evoked PSCs from tdTomato-positive dCA2 pyramidal neurons, which were completely blocked (>90%) by bath-application of NBQX (n = 13). b, Representative traces of PSCs evoked by paired-pulse MS input activation in slices prepared from Amigo2-Cre mice exposed to a novel object or novel mouse (social). Scale bars, 50 pA, 100 ms. Quantification of paired-pulse ratios for mice (below) interacting with a novel object (n = 17/3, cells/mice) or novel mouse (n = 24/4) (t39 = 1.589, P = 0.1201), two-tailed unpaired Student’s t-test. N.S. = not significant. c, Representative EPSCs from tdTomato-positive dCA2 pyramidal neurons before and after LTD induction protocol. Scale bars, 25 pA, 100 ms. Summary time-course of EPSCs with and without listed receptor antagonists in bath. With inhibitors: n = 8/4, without inhibitors: n = 6/3. d, Representative image of MS ChR2 injection site (above) and optical fibre implant site in the dCA2 (below). n = 10. Scale bars, 500 μm. Error bars denote s.e.m.
