Extended Data Fig. 8: Effects of CP93129 on dCA2-projecting MS neurons.
From: 5-HT modulation of a medial septal circuit tunes social memory stability
a, Sociability is not affected by MS infusion of indicated drugs. Duration in chamber with no or nm (left) and discrimination scores (right) (F5,78 = 0.3144, P = 0.9029; n = 14). b, Pie chart summarizing postsynaptic responses of EGFP-positive cells in CP93129 (2 or 5 μM, n = 42). c, Pie chart illustrating action potential (AP) firing changes in CP93129 (left, n = 15). Representative traces -/+ CP93129 (right). Vm refers to unclamped resting membrane potential. Scale bars, 25 mV, 100 ms. d, Effects of CP93129 on MS neurons projecting to dCA2. Number of cells per current are indicated in parentheses (t25 = 3.461, P = 0.002; n = 26) (NOCHG, no change). e, Quantification of action potential firing as function of current injection for dCA2-projecting MS neurons with increased firing in CP93129 (P = 0.001; n = 10). f, Summary time course of IPSC responses in D-AP5 and NBQX with a pipette solution comprising CsMeSO4+CsCl. Representative traces above shown at indicated time points. Scale bars, 100 pA, 50 ms, n = 5/2. g, Summary time course of EPSC responses in picrotoxin while recording with a pipette solution comprising CsMeSO4. Representative traces above shown at indicated time points. Scale bars, 50 pA, 10 ms, n = 5/3. h, Amplitudes of IPSCs (t4 = 0.0285, P = 0.979, n = 5) and EPSCs (t4 = 0.593, P = 0.585, n = 5) averaged over the last 5 min of recording as a percentage of the first 3 min of baseline recording. i, j, Corresponding input resistance measurements for cells shown in f and g. k, RN of IPSC (t4 = 0.058, P = 0.957, n = 5) and EPSC (t4 = −1.409, P = 0.232, n = 5) recordings averaged over the last 5 min of recording as a percentage of the first 3 min of baseline recording. l, Recordings of spontaneous inhibitory postsynaptic currents (sIPSCs) from EGFP-positive cells before and after bath-application of CP93129 (CP). Cumulative probability plot of sIPSC amplitudes with representative traces (above, scale bars, 15 pA, 15 ms, n = 9/6 (cells/mice)). Bar graph shows effect of CP93129 on mean IPSC amplitude. m, Cumulative probability plot of sIPSC inter-event intervals with representative traces (above, scale bars, 50 pA, 0.5 s, n = 9/6). Bar graph shows effect of CP93129 on mean IPSC frequency. Statistical tests: a, duration: two-tailed paired Student’s t-test; discrimination scores: one-way ANOVA with Tukey’s post-hoc test. d, h, k, Two-tailed paired Student’s t-test. e, Repeated measures two-way ANOVA with Sidak’s multiple comparison post-hoc test. l, m, tTwo-tailed Wilcoxon signed rank test. N.S. = not significant, *P < 0.05, **P < 0.01, ***P < 0.001. Error bars and shading denote s.e.m.
