Extended Data Fig. 9: Optogenetic inhibition or excitation of 5-HT inputs in the MS does not alter sociability and object memory.
From: 5-HT modulation of a medial septal circuit tunes social memory stability
a, Schematic of virus injection (left) and representative images of the injection site in the median raphe (MR) on the left and the EGFP-positive axons in the MS (right). n = 5. b, Schematic of virus injections to perform TRIO (left) and representative images of the injection site in the MS and the GFP-positive cells in the MR (right). n = 3. Scale bars = 500 μm. c, d, Duration in chamber with no or nm (left) and discrimination scores (right) (c: t9 = 1.834; P = 0.0998; n = 10. d: P = 0.3750; n = 10). e, f, Duration in chamber with fo or no (left) and discrimination scores (right) (e: t9 = 0.2418, P = 0.8143; n = 10. f: t8 = 0.6029, P = 0.5632, n = 9). g, h, Duration in chamber with no or nm (left) and discrimination scores (right) (g: t10 = 0.9294, P = 0.3746; n = 11. h: t9 = 1.518, P = 0.1633; n = 10). i, Duration in chamber with no or nm (left) and discrimination scores (right) (t11 = 1.52, P = 0.152; saline: n = 12, CP93129: n = 14). Statistical tests: d, eYFP on duration and discrimination score: two-tailed Wilcoxon signed rank test. All other data in this figure were analysed by two-tailed paired Student’s t-test. N.S. = not significant, *P < 0.05, **P < 0.01, ***P < 0.001. Error bars denote s.e.m.
