Extended Data Fig. 9: Elovl1 cKO validation.
From: Neurotoxic reactive astrocytes induce cell death via saturated lipids
a, GFP expression (green) from NuTrap mice crossed to Gfap-Cre line used in this study shows efficient recombination in Slc1a3+ astrocytes (red, as identified by RNAscope in situ hybridization) of the ganglion cell layer (GCL, identified by DAPI staining of nuclei, blue; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer). b, DNA gel following PCR amplification of Elovl1 and Gfap in the retina and optic nerve shows a decrease in Elovl1 expression relative to Gfap expression in the Elovl1 cKO mouse visual system. (white numbers indicate molecular weight markers) c, Quantification of the decrease in Elovl1 expression relative to Gfap expression in the Elovl1 cKO retina (top) and optic nerve (bottom) N=4 animals per group, bars represent s.e.m., two-tailed Student’s t-test). d, Targeted lipidomics of Elovl1 cKO ACM shows dampened upregulation of the long-chain saturated lipids normally upregulated in WT reactive ACM. (black line indicates equal upregulation; red dots indicate lipids less upregulated in Elovl1 cKO versus WT ACM; black dot indicates a lipid less upregulated in WT ACM versus Elovl1 cKO ACM). e, Separation of Elovl1 cKO and WT cell and ACM lipidomes in PCA space.
