Extended Data Fig. 5: Distinct output from the MOs, MOp-ul, and SSp-ul.

Related to Figs. 1, 2. Panels show brain-wide axonal projections (a–l) following injections of PHA-L (pink) into MOp-ul, AAV-RFP (red) and AAV-GFP (green) into immediately adjacent MOs and SSp-ul, respectively (injection sites shown in panel c). MOp-ul and SSp-ul project to similar cortical regions (a–e), however they differ in their projections to the thalamus and spinal cord, with SSp-ul uniquely innervating VPL (e, f) and targeting more dorsal layers of spinal cord (l), compared with MOp-ul. In addition, the MOs region just medial to MOp-ul is defined by prominent cortical projections to ORBvl (a, b), RSPd, and PTLp (f), and innervates MD and LP in thalamus (e, f), but lacks a projection to spinal cord (l). Together, these distinct projection profiles demonstrate differences in connectivity that emerge along the medial and lateral borders of MOp-ul. Acronyms are defined in Supplementary Table 1. Scale bars, 1 mm (left panels), 500 µm (right panels, a–l). Please see Supplementary Information for a detailed description of regional output from MOp-ul mapped using PHAL.