Extended Data Fig. 11: Approaches for further establishing synaptic connectivity in downstream targets of MOp. | Nature

Extended Data Fig. 11: Approaches for further establishing synaptic connectivity in downstream targets of MOp.

From: Cellular anatomy of the mouse primary motor cortex

Extended Data Fig. 11

Related to Fig. 2. a, To distinguish fluorescent labeling of synaptic boutons versus axons of passage in a given MOp target, AAV-hSyn-mRuby2-sypEGFP was injected into MOp-ul and the anterior pretectal nucleus (APN) was examined for the presence of synaptophysin-tagged EGFP+ boutons (green) and mRuby2+ axons (red). b, Injection site in MOp-ul. c, Labeling of boutons and axons in APN at 4X, 10X (d), and 40X magnification (e), confirming synaptic innervation of the downstream structure. f, Similarly, AAV1-hSyn-Cre may be used to confirm and quantify synaptic connectivity in a given target region following anterograde transsynaptic spread of the virus to downstream neurons and subsequent expression of tdTomato in Ai14 Cre-reporter mice. g, Injection site in MOp-ul. h, Post-synaptically labeled tdTomato+ cells (red) in APN at 4X, 10X (i), and 40X magnification (j) confirming a similar pattern of innervation as shown in (d). Blue, fluorescent Nissl stain. Values in mm relative to bregma. Scale bars, 1 mm (b, c, g, h), 200 µm (d, i), 50 µm (e, j).

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