Extended Data Fig. 6: Somatic mutations of FOXO1 impair its phosphorylation and nuclear export.
From: Convergent somatic mutations in metabolism genes in chronic liver disease
a, HepG2 cells were transfected with the indicated wild-type or mutant constructs of FOXO1 fused with a C-terminal GFP. Cells were counterstained with DAPI to highlight the nucleus, and imaged after overnight serum starvation conditions (left) and after 15 min of exposure to 100 nM insulin (right). Studies were performed in triplicate. b, HepG2 cells, expressing ectopic eGFP-tagged wild-type or mutant FOXO1 constructs as indicated and treated for 15 min with vehicle or insulin (100nM), were analysed for the indicated proteins by immunoblotting. Molecular weight markers (kDa) indicated. Studies were performed in triplicate. Uncropped versions of the blots are shown in Supplementary Fig. 4.
