Extended Data Fig. 8: RNA sequencing from cell lines transduced with either wild-type or mutant FOXO1-GFP constructs.
From: Convergent somatic mutations in metabolism genes in chronic liver disease
a, Heat map showing gene expression levels for genes in the ‘Canonical Glycolysis’ gene set from GO (GO:0061621). The order of genes on the x axis is determined by the level of significance (and direction of change) and the order of samples on the y axis is by condition (FOXO1 status and insulin status). b, Heat map showing gene expression levels for genes in the ‘Cell cycle, mitotic’ gene set from Reactome (R-HSA-69278). The order of genes on the x axis is determined by the level of significance (and direction of change) and the order of samples on the y axis is by condition (FOXO1 status and insulin status). c–e, Enrichment plots for the ‘FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes’ gene set of Reactome (9615017) (c); ‘Lipid catabolic process’ gene set of GO (0016042) (d); and ‘Apoptotic process’ gene set of GO (0006915) (e). In each, the top panel reflects the cumulative enrichment score as the gene set is traversed from most up-regulated to most down-regulated in the presence of FOXO1-mutant constructs. The bottom panel in each shows the ranking of each gene in the gene set across all genes measured.
