Extended Data Fig. 6: Details of Nup96-depleted NPC structures.
From: The cellular environment shapes the nuclear pore complex architecture
a, Tomographic slice from a lamella prepared by cryo-FIB of NUP96::Neon-AID DLD-1 cells in the presence of auxin for 4 h (Nup96-depleted). Arrowheads indicate Nup96-depleted NPCs. b, Orthoslices across the nucleocytoplasmic axis for the final aligned maps of the three Nup96-depleted NPC classes. c, Measurements of NPC diameter from single NPCs in each of the data sets as well as the non-auxin treated (WT). *** indicates a two-tailed p-value ≤ 0.001 and **** indicates a two-tailed p-value ≤ 0.0001 from Mann-Whitney U analysis of each distribution compared with the wild-type condition. Median values from each distribution are indicated as a dotted line and shown above the plot. d, Orthoslices across the nucleocytoplasmic axis of single NPCs in each of the three Nup96-depleted NPC classes. Slice thickness is 4.1 nm. e, Orthoslices across the nucleocytoplasmic axis of class averages after manual orientation alignment and classification. f, Fourier shell correlation curves for the Nup96-depleted NPC classes. FSC0.5 and FSC0.143 are indicated as dotted lines. g, Tomographic slice of Nup96-depleted cells showed instances of NE herniation at the ONM (example indicated by arrow). Slice thickness for all panels except d: 2.7 nm.
