Extended Data Fig. 1: Details of in situ human NPC architecture.
From: The cellular environment shapes the nuclear pore complex architecture
a, Orthoslices through the nucleocytoplasmic axis, CR, and NR for our cryo-ET map of the human NPC from FIB-milled DLD-1 cells. Slice thickness: 3.4 nm. b, Fourier shell correlation curves of the CR, IR, and NR regions in our map. FSC0.5 and FSC0.143 are indicated as dotted lines. c, The local resolution for the CR, IR, and NR protomers is visualized as surface color. d, Segment of the CR to highlight fitting of the Y complex structure and several unique features including putative Nup358 density (green arrowheads), extra unassigned density attached to the inner ring of the Y complex Nup85 (black arrowheads), and density we attribute to the Nup214 complex (blue arrowheads). e, Segment of the IR from our cryo-ET density with updated model fit inside. f, Segment of the NR to highlight fitting of the Y complex structure and several unique features including linker density between the Y complex rings (red arrowheads), unassigned density attached to the inner ring of the Y complex Nup85 (black arrowheads), and linkers between the Y complex inner ring and the IR (purple arrowheads).
