Extended Data Fig. 5: Selective killing of macrolides and tetracyclines.
From: Unravelling the collateral damage of antibiotics on gut bacteria
a. Time-kill curves. The survival of 12 abundant gut microorganisms was assessed over a 5 h-treatment with either erythromycin, azithromycin or doxycycline. The graph shows the mean±SD of 3 independent experiments. b. Live/dead staining of macrolide or tetracycline-treated E. coli ED1a and B. vulgatus. The left panel shows an overlay of phase contrast and fluorescence microscopy images of propidium iodide (PI)-stained E. coli ED1a or B. vulgatus before and 5 h after erythromycin, azithromycin or doxycycline treatment. Cultures were concentrated before imaging; the scale bar is 10 µm. The right panel shows the corresponding quantification of live/dead-stained cells by flow cytometry with Syto9 on the x-axis (live cells) and PI on the y-axis (dead cells). As E. coli ED1a cells stain poorly with Syto9, we only quantified PI stained cells in this case. Both the total number of measured events (n) and the percentage of cells found in each region of the graph are indicated.
