Extended Data Fig. 3: Cryo-EM data and image processing flow for DISP1-A and DISP1-A-NNN.
From: Dispatched uses Na+ flux to power release of lipid-modified Hedgehog
a, A representative cryo-EM micrograph (n = 4487 for DISP1-A, n = 4467 for DISP1-A-NNN) and several highly-populated, reference-free 2D class averages are shown for DISP1-A (left) and DISP1-A-NNN (right). The micrograph for DISP1-A-NNN has been contrast-stretched for display in order to account for the presence of a gold edge in the upper left corner of the image (the DISP1-A-NNN particle distribution on this grid necessitated targeting of the gold edge). b, Schematic flow-chart representing the image processing approach for DISP1-A. Thumbnail images of each 3D class or refinement are shown along with global GS-FSC resolution in black, particle counts in red, and dashed black boxes to indicate selected 3D classes. After separation of the R and T conformations in the first round of 3D classification, the identical processing flows for the two conformations are shown in parallel. Cryo-EM map (Red box) and atomic model of R conformation are used in main figures to present DISP1-A features. Subclasses of R and T conformations, R1, R2, T1 and T3, are labeled.
