Extended Data Fig. 1: VMHvlERαKO does not affect energy intake or physical activity during the light phase but does decrease iBAT thermogenic gene expression.
From: Oestrogen engages brain MC4R signalling to drive physical activity in female mice
a, Representative image of a successful hit confirmed post-mortem by loss of ERα expression (red) in the VMHvl (white arrow) with corresponding expression of GFP driven by the AAV2-Cre viral vector. Scale bars, 100 µm. b, Food intake and X-ambulatory parameters obtained in light period for VMHvlErαKO (n = 10) and ARCERαKO (n = 12) female cohorts compared to controls (n = 7 and 5). c, d, Equivalent fat mass in VMHvlERαKO (n = 9), ARCERαKO (n = 11), and control (n = 7 and 6) (c) and oxygen consumption rates in VMHvlERαKO (n = 12), ARCERαKO (n = 11), and control (n = 6 and 6) (d) female mice measured by EchoMRI and indirect calorimetry in CLAMS, respectively. e, Quantification of BAT thermogenic gene expression levels by qPCR in VMHvlERαKO (n = 6), ARCERαKO (n = 7), and control (n = 6 and 3) female mice (VMHvlERαKO Ucp1: unpaired two-tailed Student's t-test, t9 = 2.599, P = 0.0288).
