Extended Data Fig. 3: ERα-binding sites in oestradiol-benzoate-sensitive target genes contain conserved ERE consensus sequences.
From: Oestrogen engages brain MC4R signalling to drive physical activity in female mice
CUT&RUN CPM-normalized coverage track showing oestradiol-benzoate-specific ERα binding sites containing EREs (pink boxes) within the Greb1 locus (a; 3/3 replicates) and Pgr locus (b; 3/3 replicates), and in the Mc4r promoter (c; 1 of 3 replicates) in 400,000 sub-cortical brain nuclei collected from vehicle and oestradiol benzoate (5 µg) treated gonadectomized mice. Below each track the location and sequence conservation of full (a, b) ERE and half (c) SP1/ERE consensus sites in target gene loci indicated by pink and green boxes. d, e, Location and sequence conservation of ERE consensus sites within Mc4r and Nmur2 loci corresponding to ERα binding sites presented in Fig 1g. For all panels the genomic intervals containing ERE/SP1 sites are located within the ERα binding sites identified by CUT&RUN.
