Extended Data Fig. 4: Limited induction of Mc4r expression in the MeA by ERα signalling, and additional neuroanatomical targets of VMHvlMC4R neurons.
From: Oestrogen engages brain MC4R signalling to drive physical activity in female mice
a, Representative coronal brain images of Ai14Mc4r female mice stained for ERα (green) and Ai14 (magenta) in the MeA used for quantification shown in Fig 2a. b, Additional ISH comparing Mc4r induction in the VMHvl and MEA in oestrus and proestrus female mice. c, Representative mYFP reporter expression in additional neuroanatomical regions. Scale bars, 200 µm. d, Heat map from Fig. 2d rearranged to compare VMHvlMC4R and VMHvlERα projection intensity in target regions along the anterior-posterior axis. e, VMHvlMC4R projections to the PAG preferentially target the PAGdl/l and PAGdm. Scale bars, 200 µm.
