Extended Data Fig. 2: Endogenous MOPR activation in mNAcSh is necessary for potentiating state-dependent avoidance behavior.

a. Schematic of elevated zero maze (EZM) test. Mice were tested after habituation to the test room (Unrestrained) or after 30 min of restraint stress (Restraint). b. Example heat plots of time spend in the open arms of the EZM in wildtype (WT, left) or Oprm1 KO (KO, right) after no restraint (top) or 30 min of restraint (bottom). c. Unrestrained WT mice spent ~30% of the EZM test in the open arms. Mice exposed to restraint stress significantly reduced their exploration to 10%. Pretreatment with naloxone prevented Restraint induced avoidance. Oprm1 KO mice did not display open arm avoidance after Restraint. Penk-Cre x cKO mice displayed normal avoidance of the open arms after Restraint (n = 9 WT Unrestrained, 9 WT Restrained, 8 Oprm1 KO Unrestrained, 7 Oprm1 KO Restrained, 8 Oprm1^fl/fl x Penk-Cre- Restrained, 9 Oprm1^fl/fl x Penk-Cre+ Restrained). d. Schematic of food intake assay after food deprivation or Restraint. e. Food deprived mice showed normal increase in intake relative to their ad libitum test day. Mice exposed to Restraint did not increase food intake relative to their Unrestrained test day (n = 11 deprived, 10 Restrained). All error bars represent ± SEM and n = biologically independent mice. Medians marked with orange bar. Post hoc p-values are derived from Two-way ANOVA with Sidak multiple comparisons (c, e).