Extended Data Fig. 6: Validation of MORC3 as a repressor of IFN.
From: Self-guarding of MORC3 enables virulence factor-triggered immunity
(a) Schematic of a genome wide CRISPR screen to identify negative regulators of IFN. (b) PinAPL.py analysis of the CRISPR screen to identify negative regulators of IFN. Significantly enriched genes are labeled. Raw data can be found in Supplementary Table 2. (c) U2OS cells were infected with HSV-1 for 24h. One representative immunoblot of two is shown. (d, e) Gene expression of BLaER1 monocytes is shown as mean + SEM of 3 independent experiment from one representative clone or two (multiple KOs) or one clone (WT and MORC3–/–) except CXCL10 quantification in (d) which is shown as mean of 2 independent experiments. *** p < 0.001; significantly different than the corresponding WT condition, tested by two-way ANOVA and Dunnett’s post hoc test. See Source Data for exact p values. (f-i) Immunoblot of BLaER1 monocytes of indicated genotypes
