Extended Data Fig. 7: Characterization of MORC3 deficiency in THP1, U937 and BLaER1 cells.
From: Self-guarding of MORC3 enables virulence factor-triggered immunity
(a-d) Gene-expression in PMA-differentiated THP1-Cas9 or PMA-differentiated U937-Cas9 human myeloid-like cells expressing indicated sgRNAs is depicted as mean + SEM of three independent experiments. Protein expression in the same cells was analyzed by immunoblot. ND = not detected. *** p < 0.001; ns = not significantly different than the corresponding scramble sgRNA-expressing condition, tested by two-way ANOVA and Bonferroni’s post hoc test. See Source Data for exact p values. (e) Transcriptional changes in BLaER1 monocytes as detected by RNA-seq in three independent experiments are depicted by PCA. These data are partially duplicated from Extended Data Fig. 5d. (f) Modules from the Molecular Signatures Database that were found to be enriched in differentially expressed genes in MORC3–/– BLaER1 monocytes. Background gene set includes all genes that have base mean expression of at least 1 in MORC3–/– BLaER1 monocytes. Genes contributing to individual modules can be found in Supplementary Table 1
