Extended Data Fig. 8: PML is not required for IFNB1 regulation by MORC3.
From: Self-guarding of MORC3 enables virulence factor-triggered immunity
(a) Gene expression of BLaER1 monocytes of indicated genotype is depicted as mean + SEM of three independent experiments. (b) Protein expression of BLaER1 monocytes was analyzed by immunoblot. (c) BLaER1 monocytes expressing doxycycline-inducible virulence factors were stimulated with doxycycline for 24h. Gene-expression as quantified by q-RT-PCR is depicted as mean + SEM of three independent experiments. (d, e) Gene-expression in HCT116-Cas9 cells expressing indicated sgRNAs is depicted as mean + SEM of three independent experiments. Protein expression in the same cells was analyzed by immunoblot. ND = not detected. ns = not significantly different than the corresponding scramble sgRNA-expressing condition, tested by Mixed-effects model and Bonferroni’s post hoc test. See Source Data for exact p values. (f) BLaER1 monocytes of indicated genotype were infected with ΔICP0 HSV-1 at indicated MOI for 6h and analyzed by flow cytometry. Mean + SEM of three independent experiment is depicted. * p < 0.05 ; ns = not significantly different than the IFNAR1–/–IFNAR2–/– condition, tested by two-way ANOVA and Bonferroni’s post hoc test. See Source Data for exact p values
