Extended Data Fig. 11: Positional de-repression of IFNB1 explains IFN induction by virulence factors.
From: Self-guarding of MORC3 enables virulence factor-triggered immunity
(a) BLaER1 monocytes were infected with HSV-1 at MOI=1 for 24h. MLLT3 expression is depicted as mean + SEM of n = 3 independent experiments. *** p < 0.001; ns = not significantly different than mock infection, tested by two-way ANOVA and Dunnett’s post hoc test. See Source Data for exact p values. (b) Mean log transcripts per million (TPM) of a gene cluster at chromosome 9 in BLaER1 monocytes. Mean TPM is calculated for each condition across three independent experiments. All protein coding genes within this region are depicted. (c) Log transcripts per million (TPM) of IFN genes in BLaER1 monocytes upon virulence factor expression were detected by RNA-seq in three independent experiments. (d) BLaER1 monocytes expressing doxycycline-inducible virulence factors were stimulated with doxycycline for 24h. Gene expression is shown as mean + SEM of n = 3 independent experiments. ** p < 0.01; *** p < 0.001; ns = not significantly different than WT, tested by two-way ANOVA and Dunnett’s post hoc test. See Source Data for exact p values. Note that IFNB1–/– cells harbor small indels within IFNB1 that allow detection of mRNA by q-RT-PCR. (e, f) BLaER1 monocytes expressing doxycycline-inducible virulence factors were stimulated with doxycycline for 24h. Transcriptomic changes as detected by RNA-seq in three independent experiments were analyzed PCA. Data is partially duplicated from Extended Data Fig. 10i. The distance on PC1-axis between samples and the mean of mCherry-expressing cells was calculated and is depicted as mean + SEM from three independent experiments. *** p < 0.001 significantly different than WT, tested by two-way ANOVA and Dunnett’s post hoc test. See Source Data for exact p values
