Fig. 2: Two MD circuits for amplification and suppression of PFC activity.
From: Thalamic circuits for independent control of prefrontal signal and noise
a, Prefrontal PV+ and VIP+ input mapping. b, MD neurons targeting VIP+ (left) and PV+ (right) interneurons occupy distinct MDl domains. Scale bars, 200 µm. c, Group summary for location of VIP- and PV-projecting MDl neurons (n = 73 VIP-projecting (7 mice) and n = 117 PV-projecting (4 mice)). d, KNN clustering and representational similarity analysis show robust separation. e, Labelling MDD2 and MDGRIK4 neurons using the corresponding Cre lines. f, MDD2 and MDGRIK4 neurons also occupy distinct anatomical locations. Scale bars, 200 μm. g, Group summary of MDD2 and MDGRIK4 neurons in MDl (n = 177 MDD2 neurons and 194 MDGRIK4 neurons from 3 mice each). h, MDD2 and MDGRIK4 locations show additional high representational similarity to VIP- and PV-projecting neurons, respectively. i, j, mGRASP labelling shows higher innervation of VIP+ neurons by MDD2 (i; n = 21 neurons from 3 D2-cre, n = 25 neurons from 3 GRIK4-cre mice, respectively; Kolmogorov–Smirnov) and higher innervation of PV+ neurons by MDGRIK4 (j; n = 27 neurons from 3 D2-cre, n = 32 neurons from 3 GRIK4-cre mice, respectively; Kolmogorov–Smirnov). Scale bars, 3 µm. k, Hypothesized circuit. l, Selective MD cell-type activation set-up. m, MDD2 but not MDGRIK4 amplify functional PL connectivity (n = 100 and n = 68 PL neural responses from 3 mice each for MDD2 and MDGRIK4, respectively; left to right: MDD2 P = 1.0 × 10−5 for all; MDGRIK4 P = 0.0599, 0.0789, 0.0575, 0.1311 (NS) for laser powers displayed; Mann-Whitney U, compared to baseline). n, MDGRIK4 but not MDD2 suppress PL neural spike rates (n = 1,257 and n = 697 putative excitatory PL neurons from 3 mice each; MDD2 P = 0.184, 0.605, 0.579, 0.739 (NS); MDGRIK4 P = 0.298, P = 0.067, *P = 0.033, ***P = 1.61 × 10−5, respectively, for laser powers displayed; Mann-Whitney U compared to baseline). All statistical tests are two-tailed. Box plot parameters as in Fig. 1. Data are mean ± s.e.m. for m, n.
