Fig. 3: Structural basis of CARs binding to PHOX2B peptide presented on multiple HLAs.
From: RETRACTED ARTICLE: Cross-HLA targeting of intracellular oncoproteins with peptide-centric CARs
a, PHOX2B–HLA-A*24:02 crystal structure and models of PHOX2B in complex with HLA-A*23:01, HLA-B*14:02 and HLA-C*07:02. b, The charged and polar R151, Q155 and R69 residues of HLA-C*07:02 align with key 10LH interaction residues I5, R6 and I7 (MHC residues in blue and PHOX2B–10LH interaction residues in red). R151, Q155 and R69 create steric and charged hindrance of key peptide-binding residues. c, Staining of the PHOX2B PC-CAR 10LH reveals strong binding to HLA-A*24:02, HLA-A*23:01 and HLA-B*14:02, but not to HLA-C*07:02. CD19, CD19-directed CAR; 10LH, PHOX2B PC-CAR; UT, untransduced T cells. Created with BioRender.com.
