Extended Data Fig. 2: Validation of antigens discovered by immunopeptidomics using LC/MS/MS of synthetic peptide.
From: RETRACTED ARTICLE: Cross-HLA targeting of intracellular oncoproteins with peptide-centric CARs
Peptide sequences imputed from immunopeptidomics spectra were synthesized and LC/MS/MS was under matched conditions. Synthetic peptides show complete concordance with tumor-eluted peptides across all b and y ions; masses highlighted in green represent detected peaks corresponding to matched b and y ions found in both tumor-eluted peptide spectra and synthetic peptide spectra.
