Extended Data Fig. 8: Enrichment of 15N in amino acids.
From: Terrestrial-type nitrogen-fixing symbiosis between seagrass and a marine bacterium
Enrichment of 15N in individual amino acids relative to the enrichment of 15N in glutamate, the first amino acid synthesized upon N2 fixation, in root-extracted proteins after incubations of plants with 15N2 (Methods; up to four plants that yielded sufficient amino acids for δ15N measurement are shown here). a) Amino acids synthesized from intermediates of the glycolysis or the pentose phosphate pathway and b) amino acids synthesized from intermediates of the TCA cycle or via transamination of another amino acid (or intermediates) with glutamate. Slopes of the linear regressions are given behind the three-letter code for each amino acid. A slope of 1.0 indicates 15N enrichments that are identical to those of glutamate with smaller slopes indicating lower 15N enrichments (see Supplementary Discussion). Phe, phenylalanine (R2=0.991); Leu, Leucine (R2=0.995); Val, Valine (R2=0.993); Ser, Serine (R2 = 0.998); Ala, Alanine (R2 = 0.819); Lys, Lysine (R2 = 0.979); Thr, Threonine (R2 = 0.986); Pro, Proline (R2 = 0.988); Gly, Glycine (R2 = 0.992); Asp, Aspartate (R2 = 0.999).
