Extended Data Fig. 5: STEM and epifluorescence images of seagrass root sections.
From: Terrestrial-type nitrogen-fixing symbiosis between seagrass and a marine bacterium
(a, b) STEM images with black arrowheads indicating bacteria residing in intercellular space (a) as well as inside plant cells (b). Based on the location as well as the relative and absolute abundances of Ca. C. neptuna in root cross-sections (Extended Data Fig. 4), the majority of bacteria visible are likely Ca. C. neptuna cells. (c) Epifluorescence image of a root cross-section showing Ca. C. neptuna cells (overlay image of DAPI (blue), autofluorescence (green/orange) and FISH-positive cells (pinkish color due to the overlap of DAPI and FISH probe (orange) signals) inside the stele.
