Extended Data Fig. 7: Proximal dendritic properties and additional distal dendritic properties. Related to Fig. 2.
From: Allometric rules for mammalian cortical layer 5 neuron biophysics
a, Two-photon z-stack montage image of mouse neuron with a proximal patch-clamp electrode 63 μm from soma. b, Proximal dendritic voltage in response to subthreshold (top) or threshold (bottom) step current injections. c-d, Subthreshold properties of proximal dendrites (mouse n = 31, gerbil n = 23, rat n = 19, guinea pig n = 28, rabbit n = 21, human n = 27). Box plots denote the median and 25–75th percentiles. c, Proximal input resistance (p < 10−18 Kruskal-Wallis, χ2 = 98 & 5 df). d, Proximal voltage sag (p < 10−8 Kruskal-Wallis, χ2 = 47 & 5 df). e-i, Suprathreshold properties of proximal dendrites (mouse n = 19, gerbil n = 20, rat n = 9, guinea pig n = 18, rabbit n = 12, human n = 15). Box plots denote the median and 25–75th percentiles. e, Proximal rheobase (p < 10−9 Kruskal-Wallis, χ2 = 51 & 5 df). Data points displayed as a beeswarm plot to show overlapping integers. f, Proximal spike threshold (p < 10−4 Kruskal-Wallis, χ2 = 31 & 5 df). g, Proximal spike area on a log scale (p < 10−6 Kruskal-Wallis, χ2 = 36 & 5 df). h, Proximal spike width on a log scale (p < 10−5 Kruskal-Wallis, χ2 = 36 & 5 df). i, Proximal maximum spike dV/dt (p < 10−5 Kruskal-Wallis, χ2 = 35 & 5 df). j-m, Additional suprathreshold properties of distal dendrites (mouse n = 18, gerbil n = 19, rat n = 47, guinea pig n = 25, rabbit n = 20, human n = 25). Box plots denote the median and 25–75th percentiles. j, Distal rheobase (p < 10−4 Kruskal-Wallis, χ2 = 28 & 5 df). Data points displayed as a beeswarm plot to show overlapping integers. k, Distal spike threshold (p < 10−11 Kruskal-Wallis, χ2 = 61 & 5 df). l, Distal spike area on a log scale (p < 10−10 Kruskal-Wallis, χ2 = 58 & 5 df). m, Maximum distal spike dV/dt (p < 10−13 Kruskal-Wallis, χ2 = 74 & 5 df). n-o, Somatic outside-out currents in sclerosis (n = 44), tumour (n = 30) and others (n = 12). Box plots denote the median and 25–75th percentiles. n, Somatic Kv peak currents (p = 0.39 Kruskal-Wallis, χ2 = 1.90 & 2 df). o, Somatic Kv plateau currents (p = 0.35 Kruskal-Wallis, χ2 = 2.09 & 2 df). p, Example EEG recording of epileptic seizure in rat kainic acid model. q-r, Somatic outside-out currents in control (n = 80) and epileptic (n = 68) rats. Box plots denote the median and 25–75th percentiles. q, Somatic Kv peak currents (p = 0.65, two-sided Wilcoxon rank sum, Z = 0.45). r, Somatic Kv plateau currents (p = 0.525, two-sided Wilcoxon rank sum, Z = 0.64).
