Extended Data Fig. 8: Additional conductance measurements. Related to Fig. 3.
From: Allometric rules for mammalian cortical layer 5 neuron biophysics
a, Dendritic outside-out patches were pulled from proximal dendrites after obtaining whole-cell recordings. Top, HCN currents with the associated voltage-clamp protocol on the left. Bottom, Kv currents with the associated voltage-clamp protocol on the left. b-g, Box plots denote the median and 25–75th percentiles. b, Proximal Kv peak currents (p = 0.010 Kruskal-Wallis, χ2 = 15 & 5 df; mouse n = 32, gerbil n = 38, rat n = 38, guinea pig n = 37, rabbit n = 35, human n = 44). c, Proximal HCN steady-state currents (p=0.07 Kruskal-Wallis, χ2 = 10 & 5 df; mouse n = 29, gerbil n = 30, rat n = 38, guinea pig n = 30, rabbit n = 31, human n = 42). d, Somatic Kv peak currents (p < 10-19 Kruskal-Wallis, χ2 = 115 & 9 df; Etruscan shrew n = 59, mouse n = 56, gerbil n = 80, rat n = 80, ferret n = 80, guinea pig n = 70, rabbit n = 53, marmoset n = 63, macaque n = 87, human n = 86). e, Somatic Kv plateau currents (p < 10-19 Kruskal-Wallis, χ2 = 115 & 9 df; Etruscan shrew n = 59, mouse n = 56, gerbil n = 80, rat n = 80, ferret n = 80, guinea pig n = 70, rabbit n = 53, marmoset n = 63, macaque n = 87, human n = 86). f, Proximal Kv plateau currents (p < 10-4 Kruskal-Wallis, χ2 = 29 & 5 df; mouse n = 32, gerbil n = 38, rat n = 38, guinea pig n = 26, rabbit n = 35, human n = 44). g, Distal Kv plateau currents (p =0.00001 Kruskal-Wallis, χ2 = 30 & 5 df; mouse n = 35, gerbil. n = 46, rat n = 59, guinea pig n = 58, rabbit n = 39, human n = 43). h-k, Conductance as a function of neuron size. The lines and shaded error bars represent the fit and 95% confidence interval of an allometric relationship constructed excluding humans. h, Total Kv plateau conductance on a log-log scale (exponent 1.24 ± 0.09, R2 = 0.983, p < 10-3, linear regression on log-log scale, F = 176 & 3 df, n = 5 for mouse, gerbil, rat, guinea pig, and rabbit). i, Somatic Kv plateau conductance on a log-log scale (exponent 1.98 ± 0.15, R2 = 0.962, p < 10-5, linear regression on log-log scale, F = 175 & 7 df, n = 9 for shrew, mouse, gerbil, rat, ferret, guinea pig, rabbit, marmoset, and macaque). j, Normalized average of HCN, Kv peak and Kv plateau conductance (exponent 1.43 ± 0.15, R2 = 0.966, p = 0.003, linear regression on log-log scale, F = 85.7 & 3 df, n = 5 for mouse, gerbil, rat, guinea pig, and rabbit). k, Normalized average of somatic Kv peak and Kv plateau conductance (exponent 1.82 ± 0.12, R2 = 0.968, p < 10-5, linear regression on log-log scale, F = 212 & 7 df, n = 9 for shrew, mouse, gerbil, rat, ferret, guinea pig, rabbit, marmoset and macaque). l, Relationship between somatic (normalized average of somatic Kv peak and Kv plateau conductance) and dendritic (normalized average of HCN, Kv peak and Kv plateau conductance) conductance (R2 = 0.891, p = 0.005, linear regression, F = 32.7 & 4 df, n = 6 for mouse, gerbil, rat, guinea pig, rabbit, and human). m, Soma volume as a function of neuronal density (Extended Data Table 1) on a log-log scale (exponent -0.74 ± 0.10, R2 = 0.897, p < 10-3, linear regression, F = 52.3 & 6 df, n = 8 for shrew, mouse, rat, ferret, guinea pig, rabbit, marmoset, and macaque). The line and shaded error bars represent the fit and 95% confidence interval of an allometric relationship constructed excluding humans. n-t, Allometric relationship on a log-log scale. The lines and shaded error bars represent the fit and 95% confidence interval of the relationship constructed excluding humans. n-o, Somatic Kv plateau conductance densities in volumes as in Fig. 4c. n, Membrane conductance density (exponent 0.98 ± 0.15, R2 = 0.860, p < 10-3, linear regression, F = 43.0 & 7 df, n = 9 for shrew, mouse, gerbil, rat, ferret, guinea pig, rabbit, marmoset, and macaque). o, Volume Kv peak conductance density where the volume is filled with somas (exponent 0.53 ±0.15, R2 = 0.651, p = 0.009, linear regression, F = 13.1 & 7 df, n = 9 for shrew, mouse, gerbil, rat, ferret, guinea pig, rabbit, marmoset, and macaque). p-q, Somatic Kv plateau conductance densities in volumes as in Fig. 4g. Gerbils were not included because the necessary information was not available in the literature (Extended Data Table 1). p, Cortex conductance density with accurate neuronal densities (exponent -0.27 ± 0.34, R2 = 0.092, p = 0.47, linear regression, F = 0.61 & 6 df, n = 8 for shrew, mouse, rat, ferret, guinea pig, rabbit, marmoset, and macaque). q, Total cortex conductance (exponent 1.09 ± 0.06, R2 = 0.985, p < 10-5, linear regression, F = 393 & 6 df, n = 8 for shrew, mouse, rat, ferret, guinea pig, rabbit, marmoset and macaque). r-t, Same analysis as in Fig. 4f, but including dendrites in the volume and conductance calculation. r, Volume Kv peak conductance density where the volume is filled with somas and dendrites (exponent 0.05 ±0.10, R2 = 0.083, p = 0.64, linear regression, F = 0.272 & 3 df, n = 5 for mouse, gerbil, rat, guinea pig, and rabbit). s, Volume Kv plateau conductance density where the volume is filled with somas and dendrites (exponent 0.02 ±0.13, R2 = 0.006, p = 0.90, linear regression, F = 0.02 & 3 df, n = 5 for mouse, gerbil, rat, guinea pig, and rabbit). t, Volume HCN conductance density where the volume is filled with somas and dendrites (exponent 0.5 ±0.29, R2 = 0.500, p = 0.18, linear regression, F = 2.99 & 3 df, n = 5 for mouse, gerbil, rat, guinea pig, and rabbit).
