Extended Data Fig. 6: GABA increases the proliferation, survival and bioenergetics of macrophages differentiated in vitro.
From: B cell-derived GABA elicits IL-10+ macrophages to limit anti-tumour immunity
a, Scheme of mouse bone marrow (BM)-derived monocytes (Mus-mo), total BM cell or human blood-derived monocytes (Hu-mo) differentiation in the presence of M-CSF without (M-0) or with GABA (GABA-M-0). b, The cell number and the ratio of FRβ+ cells in mouse (n = 2 (M-0 (middle), 4 (GABA-M-0 (left) or 3) or human monocytes-derived M-0 (n = 5) with or without GABA treatment. c, Quantification of the frequencies of propidium iodide (PI)+ or –, annexin V+ (n = 4) and FRβ+ cells (n = 3) by flow cytometry in mouse BM-derived M-0 cells conditioned with or without GABA. d, Transcripts related to cell cycle and folate metabolism obtained from gene chip analysis of M-0 and GABA-M-0 and represented relative to M-0 (n = 2). e, Real-time oxygen consumption rate (OCR), maximal respiratory capacity (MRC), and extracellular acidification rate (ECAR) as assessed by Seahorse assay. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (two-tailed unpaired t-test). Bars represent mean ±SEM. n indicates biological (d) or technical replicates. Data are representative of three experiments (c, e). Exact P values are in Source Data. The image of immune cell (Extended Figures) and mouse (Fig. 1a) was created with BioRender.com.
