Extended Data Fig. 10: Enhanced effector properties of CD8⁺ T cells infiltrating the tumors in Mb1^cre/+;Gad1^fl/fl mice and visualization of GABA in human renal cell cancer infiltrated with B cells and IgA⁺ plasma cells.

a, Intracellular GABA quantitation measured by mass spectrometry in FACS purified T or B cells from the LN of naïve Mb1^cre/+;Gad1^fl/+ or Mb1^cre/+;Gad1^fl/fl mice (n = 6 from 3 biologically independent mice). b, The numbers of CD8⁺ T cells or frequencies of CD8⁺ T cells in lymphocyte gate and of granzyme B⁺perforin⁺, IFNγ⁺TNFα⁺ and TNFα⁺IL-2⁺ in CD8⁺ T cell gate cells isolated from tumors of Mb1^cre/+;Gad1^fl/+ (n = 4) or Mb1^cre/+;Gad1^fl/fl (n = 5) mice. c, Imaging mass spectrometry of GABA and immunohistochemical analyses of human renal cell cancer tissue sections. Scale bar, 1 mm. ns: not significant, *P < 0.05, **P < 0.01, ****P < 0.0001 (two-tailed unpaired t-test). Bars represent mean ±SEM. n indicates biological replicates (b). The experiment is performed once (c). Exact P values are in Source Data.

Source data