Extended Data Fig. 5: HPLC-HR–MS and HPLC-HR–MS/MS analyses of acarbose-O6A-phosphate produced by AcbK and Mak1.
From: The human microbiome encodes resistance to the antidiabetic drug acarbose
Extracted Ion Chromatograms (EICs) of acarbose-O6A-phosphate produced by AcbK (a), Mak1 (d), and a co-injection of the two (f), indicating that their products are identical. EIC is displayed for m/z = 726.2-726.3 [M+H]+ in all cases. b, c, e, g, HR-MS of the two acarbose-O6A-phosphate peaks produced by either AcbK (b, c) or Mak1 (e, g), following the numbering scheme on the individual EIC peaks. h, i, identical HR-MS/MS fragmentation patterns of the two EIC peaks of acarbose-O6A-phosphate from the co-injection analysis in f. The structure of acarbose-O6A-phosphate is shown on the right, along with its predicted fragments and their calculated mass to charge ratios that match observed ones.
