Extended Data Fig. 6: Somatic hypermutation at the JH4 intron (JH4i).
From: Fam72a enforces error-prone DNA repair during antibody diversification
a, Flow cytometry analysis of germinal center B cells (B220+ Fas+ GL-7+) isolated from the Peyer’s patches of unimmunized Fam72a+/+ and Fam72a-/- mice. Plots are gated on B220+ cells. b, Distribution of mutations at the JH4 intron (JH4i) in Fam72a+/+ (top) and Fam72a-/- (bottom) sequences obtained from germinal center B cells (B220+ Fas+ GL-7+) isolated from the Peyer’s patches (PPs) of unimmunized Fam72a+/+ and Fam72a-/- mice. c, Mean frequencies (at JH4i) of transition and transversion mutations at G/C and A/T base pairs, per sequence for each individual mouse analyzed. Data come from 5 mice of each genotype. p-value was determined using two-tailed Student’s t-test. Horizontal line: mean values
