Extended Data Fig. 3: Robust B cell development and switch region transcription in Fam72a-/- mice.
From: Fam72a enforces error-prone DNA repair during antibody diversification
a, Schematic representation of the murine Fam72a locus and strategy for the generation of Fam72a-/- mouse model. b, c, Flow cytometry and cellular analysis of B cell populations in the bone marrow (b) or the spleen (c) from Fam72a+/+ and Fam72a-/- mice, using the indicated cell surface markers. The data are representative of 5 mice per genotype. Horizontal line: mean values. d, Real-time qPCR analysis for germline transcripts at donor (GLTμ) and acceptor switch regions (GLTγ3, GLTγ1, GLTγ2b, GLTγ2a and GLTα) in Fam72a+/+ and Fam72a-/- splenic B cells cultured for 96h as in c. Expression is normalized to Igβ and is presented relative to expression in Fam72a+/+ B cells, set as 1. Mean of three mice per genotype + SEM were calculated following the rules for error propagation while calculating a ratio. Statistical analysis was performed using two-tailed Student’s t-test
