Extended Data Fig. 2: NADase activity of ThsA after addition of standard cADPR.
From: Antiviral activity of bacterial TIR domains via immune signalling molecules
NADase activity of ThsA, when incubated with filtered cell lysates derived from ThsB-expressing cells 70-min post infection by phage SPO1, or with buffer containing 0 µM – 1000 µM of synthetic cyclic ADP-ribose (cADPR). NADase activity was calculated as the rate of change in εNAD fluorescence during the linear phase of the reaction. Bars represent mean of three experiments, with individual data points overlaid. NADase activity between the cADPR-containing samples and the blank sample (0 µM cADPR) are not statistically significant (One-way ANOVA, followed by pairwise multiple comparison analysis according to Tukey’s honest significant difference).
