Extended Data Fig. 11: Triple-view 1D SIM of C. elegans embryo.

a) Fixed C. elegans embryo (strain DCR6681) with tubulin immunolabelled with α-alpha tubulin primary, α-mouse-biotin, Streptavidin Alexa Fluor 568, imaged via lower View C (left) and triple-view 1D SIM mode (right). Lateral (left) and axial (right) maximum intensity projections are shown in each case. Scale bars: 5 μm. Higher magnification lateral b) views and axial c) views of yellow and red rectangular regions in a) are also indicated, highlighting progressive improvement in resolution from view C (left), to triple-view diffraction-limited result (middle), to triple-view 1D SIM result (right). Scale bars: 2 μm.