Extended Data Fig. 16: Isotropic in-plane super-resolution imaging of living cells.

a) Lateral maximum intensity projections of Jurkat T cell expressing EMTB-3XGFP (yellow) and F-tractin-tdTomato (red), volumetrically imaged every 2 s, as imaged with raw line confocal input (view C, left) and 2D SIM output after deep learning and joint deconvolution with six rotations. Four time points from 150 volume series are shown; green arrows highlight features better resolved in 2D SIM output vs. raw input; white arrows indicate actin dynamics at cell periphery. Scale bar: 5 μm. See also Supplementary Video 6. b) Imaris 3D renderings of Jurkat T cell (same sample as shown in Fig. 4c) with nucleus (histone H2B-GFP, cyan, top) and segmented centrosome (EMTB-mCherry, magenta, bottom) at indicated time points. The centrosome produces a concave nuclear deformation (red arrows), pulling and rotating the nucleus as it becomes docked at the immune synapse. Scale bar: 5 μm. c) Maximum intensity projections over bottom half of EMTB-mCherry volumes at indicated times, showing coordinated movement of the microtubule cytoskeleton. Coloured arrowheads mark regions for comparison. See also Supplementary Video 6. Scale bar: 5 μm.