Extended Data Fig. 18: Multiview super-resolution imaging of larval worm.

a) Maximum intensity projection of fixed L2 stage larval worm expressing membrane targeted GFP primarily in the nervous system, imaged in triple-view 2D SIM mode. Anatomy as highlighted. b) Higher magnification views (single slices 6 μm into volume) of dashed green rectangle in a), highlighting VNC neurons as viewed in diffraction-limited View C (upper), triple-view 1D SIM obtained by processing 15 volumes (5 per view, middle), and triple-view 2D SIM mode (3 volumes, 1 per view, lower). c) Line profiles corresponding to b1–b3 in b). d) Lateral (upper) and axial (bottom) maximum intensity projections from anaesthetized L4 stage larval worm expressing the same marker, comparing dense nerve ring region imaged in diffraction-limited view C (left), view C 2D SIM mode (middle), and iSIM (right). Purple, red arrows highlight labelled cell bodies or membranous protrusions for comparison. See also Fig. 4i–m. e) Fixed C. elegans L2 larvae (strain DCR6681) expressing GFP-membrane marker imaged in commercial OMX 3D SIM system. A single slice ~2 μm from the bottom surface of the worm is shown, derived from 5 μm stack. Raw data (top) and reconstruction (bottom) are shown. No modulation is evident in raw data, and reconstruction shows obvious artifacts (red arrows). Scale bars: a, d) 10 μm; b, e) 5 μm.