Extended Data Fig. 2: Further characterization of imaging field.
a) 175 × 175 μm2 imaging field, showing representative horizontal illumination lines across field, as measured in fluorescent dye. Also superimposed are example regions of interest (#1–#9) from a separate experiment, showing images of 100 nm fluorescent beads at different field locations. Scale bar: 20 μm. b) Excitation uniformity measured along the long axis of illumination, from lines at top, middle, and bottom of imaging field, as marked in a). c) Full width at half maximum (FWHM) of short axis of illumination line, as measured at regions in a). FWHMs are estimated by scanning illumination relative to bead and recording intensity as a function of scan position. Means and standard deviations derived from 15 beads are reported at each location. d) As in c), but now reporting x, y, and z FWHMs derived from images of individual beads (n = 15).
